CHROMATOGRAPHY
Introducing you to paper chromatography Where its... spot, elute and treat with N, H, 3. With H2S you'll pollute it With spots you'll be saluted A qualitative analysis with simplicity!
... Enjoy the.... CHROMATOGRAPHY TUTORIAL There is a....... VIDEO Return to the...... Chem 113 SYLLABUS Return to the...... PRELAB
BASIC PRINCIPLES
Chromatography is a separation technique. It physically separates a mixture into its individual components.
Chromatography is also be useful for identification. Each component moves a characteristic distance and can be identified by color or other property.
The mixture to be separated is put on an immobile matrix such as a solid or grease. This is called the..... stationary phase.
A fluid, liquid or gas, is passed over or through this stationary phase, always only going in one direction. This is called the..... mobile phase.
The components of the mixture are adsorbed on, dissolved in or absorbed by the stationary phase.
The components of the mixture are also dissolved, to varying extents, by the mobile phase.
As the mobile phase passes through the stationary phase, it picks up the components of the mixture by dissolving them. But the stationary phase also competes for the mixture's components by absorbing, adsorbing or dissolving them.
As the chromatogram elutes,
and S E P A R A T I O N occurs!
Once separated, the presence or absence of a component can be determined. For a given chromatography system, the relative position of any component remains constant. Furthermore once separated, the identity of any component can be confirmed by it physical and chemical properties.
Visit the PAPER CHROMATOGRAPHY page
© RWK
